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Migration channels produced by laser ablation for substrate endothelialization

Identifieur interne : 000732 ( Main/Exploration ); précédent : 000731; suivant : 000733

Migration channels produced by laser ablation for substrate endothelialization

Auteurs : R. Major [Pologne] ; K. Maksymow [Pologne] ; J. Marczak [Pologne] ; J. M. Lackner [Autriche] ; M. Kot [Pologne] ; B. Major [Pologne]

Source :

Mots-clés :

Abstract

Seeding of cells on functional, biocompatible scaffolds is a crucial step in achievement the desired engineered tissue. In the present study, a pulsed laser modification onto inorganic substrate was made to promote endothelium cells migration and spread. Presented scaffolds were fabricated on carbon and titanium based coatings. Fabricated films provided very good mechanical properties together with a chemical stability preservation. The substrate modification consisted of grid-like template fabrication of micrometer size meshes. The microstructure analysis of laser traces revealed the grain size increase in the zone of laser beam interaction, which exerts an influence on a surface topography. Endothelium cells locomotion was observed within 10 day time period. As a result it was shown that the modified area enhanced cells adhesion with a preferred static behavior. The performed research work improved our understanding on the pulsed laser ablation process and template size influence on cells spatial arrangement. It constituted an important step towards fabrication of inorganic, biocompatible scaffolds for successful substrate endothelialization.


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DOI: 10.2478/v10175-012-0045-2


Affiliations:


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