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Characterization of the glycinergic input to bipolar cells of the mouse retina

Identifieur interne : 001268 ( Main/Corpus ); précédent : 001267; suivant : 001269

Characterization of the glycinergic input to bipolar cells of the mouse retina

Auteurs : Elena Ivanova ; Ulrike Müller ; Heinz W Ssle

Source :

RBID : ISTEX:C55C3672B7F62719CA6978EAB0488E5B18165E74

English descriptors

Abstract

Glycine and γ‐aminobutyric acid (GABA) are the major inhibitory transmitters of the mammalian retina, and bipolar cells receive GABAergic and glycinergic inhibition from multiple amacrine cell types. Here we evaluated the functional properties and subunit composition of glycine receptors (GlyRs) in bipolar cells. Patch‐clamp recordings were performed from retinal slices of wild‐type, GlyRα1‐deficient (Glra1spd‐ot) and GlyRα3‐deficient (Glra3–/–) mice. Whole‐cell currents following glycine application and spontaneous inhibitory postsynaptic currents (IPSCs) were analysed. During the recordings the cells were filled with Alexa 488 and, thus, unequivocally identified. Glycine‐induced currents of bipolar cells were picrotoxinin‐insensitive and thus represent heteromeric channels composed of α and β subunits. Glycine‐induced currents and IPSCs were absent from all bipolar cells of Glra1spd‐ot mice, indicating that GlyRα1 is an essential subunit of bipolar cell GlyRs. By comparing IPSCs of bipolar cells in wild‐type and Glra3–/– mice, no statistically significant differences were found. OFF‐cone bipolar (CB) cells receive a strong glycinergic input from AII amacrine cells, that is preferentially based on the fast α1β‐containing channels (mean decay time constant τ = 5.9 ± 1.4 ms). We did not observe glycinergic IPSCs in ON‐CB cells and could elicit only small, if any, glycinergic currents. Rod bipolar cells receive a prominent glycinergic input that is mainly mediated by α1β‐containing channels (τ = 5.5 ± 1.6 ms). Slow IPSCs, the characteristic of GlyRs containing the α2 subunit, were not observed in bipolar cells. Thus, different bipolar cell types receive kinetically fast glycinergic inputs, preferentially mediated by GlyRs composed of α1 and β subunits.

Url:
DOI: 10.1111/j.1460-9568.2005.04557.x

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ISTEX:C55C3672B7F62719CA6978EAB0488E5B18165E74

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<i>–/–</i>
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mice, no statistically significant differences were found. OFF‐cone bipolar (CB) cells receive a strong glycinergic input from AII amacrine cells, that is preferentially based on the fast α1β‐containing channels (mean decay time constant τ = 5.9 ± 1.4 ms). We did not observe glycinergic IPSCs in ON‐CB cells and could elicit only small, if any, glycinergic currents. Rod bipolar cells receive a prominent glycinergic input that is mainly mediated by α1β‐containing channels (τ = 5.5 ± 1.6 ms). Slow IPSCs, the characteristic of GlyRs containing the α2 subunit, were not observed in bipolar cells. Thus, different bipolar cell types receive kinetically fast glycinergic inputs, preferentially mediated by GlyRs composed of α1 and β subunits.</p>
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<title>Characterization of the glycinergic input to bipolar cells of the mouse retina</title>
</titleInfo>
<titleInfo type="abbreviated">
<title>Bipolar cell glycinergic input</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA" lang="en">
<title>Characterization of the glycinergic input to bipolar cells of the mouse retina</title>
</titleInfo>
<name type="personal">
<namePart type="given">Elena</namePart>
<namePart type="family">Ivanova</namePart>
<affiliation>Department Neuroanatomy, Max‐Planck‐Institute for Brain Research, Frankfurt/Main, Germany</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Ulrike</namePart>
<namePart type="family">Müller</namePart>
<affiliation>Department Neurochemistry, Max‐Planck‐Institute for Brain Research, Frankfurt/Main, Germany</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Heinz</namePart>
<namePart type="family">Wässle</namePart>
<affiliation>Department Neuroanatomy, Max‐Planck‐Institute for Brain Research, Frankfurt/Main, Germany</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
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<publisher>Blackwell Publishing Ltd</publisher>
<place>
<placeTerm type="text">Oxford, UK</placeTerm>
</place>
<dateIssued encoding="w3cdtf">2006-01</dateIssued>
<edition>Received 26 September 2005, revised 10 November 2005, accepted 15 November 2005</edition>
<copyrightDate encoding="w3cdtf">2006</copyrightDate>
</originInfo>
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<languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<extent unit="words">12290</extent>
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<abstract lang="en">Glycine and γ‐aminobutyric acid (GABA) are the major inhibitory transmitters of the mammalian retina, and bipolar cells receive GABAergic and glycinergic inhibition from multiple amacrine cell types. Here we evaluated the functional properties and subunit composition of glycine receptors (GlyRs) in bipolar cells. Patch‐clamp recordings were performed from retinal slices of wild‐type, GlyRα1‐deficient (Glra1spd‐ot) and GlyRα3‐deficient (Glra3–/–) mice. Whole‐cell currents following glycine application and spontaneous inhibitory postsynaptic currents (IPSCs) were analysed. During the recordings the cells were filled with Alexa 488 and, thus, unequivocally identified. Glycine‐induced currents of bipolar cells were picrotoxinin‐insensitive and thus represent heteromeric channels composed of α and β subunits. Glycine‐induced currents and IPSCs were absent from all bipolar cells of Glra1spd‐ot mice, indicating that GlyRα1 is an essential subunit of bipolar cell GlyRs. By comparing IPSCs of bipolar cells in wild‐type and Glra3–/– mice, no statistically significant differences were found. OFF‐cone bipolar (CB) cells receive a strong glycinergic input from AII amacrine cells, that is preferentially based on the fast α1β‐containing channels (mean decay time constant τ = 5.9 ± 1.4 ms). We did not observe glycinergic IPSCs in ON‐CB cells and could elicit only small, if any, glycinergic currents. Rod bipolar cells receive a prominent glycinergic input that is mainly mediated by α1β‐containing channels (τ = 5.5 ± 1.6 ms). Slow IPSCs, the characteristic of GlyRs containing the α2 subunit, were not observed in bipolar cells. Thus, different bipolar cell types receive kinetically fast glycinergic inputs, preferentially mediated by GlyRs composed of α1 and β subunits.</abstract>
<subject lang="en">
<genre>Keywords</genre>
<topic>Glra1 spd‐ot mouse</topic>
<topic>Glra3–/– mouse</topic>
<topic>glycine receptors</topic>
<topic>glycinergic IPSCs</topic>
<topic>retina</topic>
</subject>
<relatedItem type="host">
<titleInfo>
<title>European Journal of Neuroscience</title>
</titleInfo>
<genre type="Journal">journal</genre>
<identifier type="ISSN">0953-816X</identifier>
<identifier type="eISSN">1460-9568</identifier>
<identifier type="DOI">10.1111/(ISSN)1460-9568</identifier>
<identifier type="PublisherID">EJN</identifier>
<part>
<date>2006</date>
<detail type="volume">
<caption>vol.</caption>
<number>23</number>
</detail>
<detail type="issue">
<caption>no.</caption>
<number>2</number>
</detail>
<extent unit="pages">
<start>350</start>
<end>364</end>
<total>15</total>
</extent>
</part>
</relatedItem>
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<identifier type="DOI">10.1111/j.1460-9568.2005.04557.x</identifier>
<identifier type="ArticleID">EJN4557</identifier>
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<recordOrigin>Blackwell Publishing Ltd</recordOrigin>
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