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Long‐term, noninvasive imaging of regulated gene expression in living mice

Identifieur interne : 001054 ( Main/Corpus ); précédent : 001053; suivant : 001055

Long‐term, noninvasive imaging of regulated gene expression in living mice

Auteurs : Mazahir T. Hasan ; Kai Schönig ; Stefan Berger ; Walter Graewe ; Hermann Bujard

Source :

RBID : ISTEX:F023DFDE4CFF32F55FA4033B936EC624163ED56D

English descriptors

Abstract

Summary: We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long‐term studies of gene function in individual mice. genesis 29:116–122, 2001. © 2001 Wiley‐Liss, Inc.

Url:
DOI: 10.1002/gene.1014

Links to Exploration step

ISTEX:F023DFDE4CFF32F55FA4033B936EC624163ED56D

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<namePart type="given">Mazahir T.</namePart>
<namePart type="family">Hasan</namePart>
<affiliation>Zentrum für Molekulare Biologie der Universitaet Heidelberg (ZMBH), Heidelberg, Germany</affiliation>
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<name type="personal">
<namePart type="given">Kai</namePart>
<namePart type="family">Schönig</namePart>
<affiliation>Zentrum für Molekulare Biologie der Universitaet Heidelberg (ZMBH), Heidelberg, Germany</affiliation>
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<name type="personal">
<namePart type="given">Stefan</namePart>
<namePart type="family">Berger</namePart>
<affiliation>Zentrum für Molekulare Biologie der Universitaet Heidelberg (ZMBH), Heidelberg, Germany</affiliation>
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<name type="personal">
<namePart type="given">Walter</namePart>
<namePart type="family">Graewe</namePart>
<affiliation>Hamamatsu Photonics Deutschland GmbH, Herrsching, Germany</affiliation>
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<name type="personal">
<namePart type="given">Hermann</namePart>
<namePart type="family">Bujard</namePart>
<affiliation>Zentrum für Molekulare Biologie der Universitaet Heidelberg (ZMBH), Heidelberg, Germany</affiliation>
<description>Correspondence: ZMBH, Universitaet Heidelberg, Im Neuenheimer Feld 282, D‐69120 Heidelberg, Germany</description>
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<publisher>John Wiley & Sons, Inc.</publisher>
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<dateIssued encoding="w3cdtf">2001-03</dateIssued>
<dateCaptured encoding="w3cdtf">2000-07-17</dateCaptured>
<dateValid encoding="w3cdtf">2000-12-30</dateValid>
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<abstract lang="en">Summary: We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long‐term studies of gene function in individual mice. genesis 29:116–122, 2001. © 2001 Wiley‐Liss, Inc.</abstract>
<note type="funding">Volkswagen‐Stiftung</note>
<note type="funding">BMBF BioRegio‐Programm</note>
<note type="funding">Fonds der Chemischen Industrie Deutschlands</note>
<subject lang="en">
<genre>Keywords</genre>
<topic>tetracycline</topic>
<topic>regulatable transcription</topic>
<topic>Cre recombinase</topic>
<topic>firefly luciferase</topic>
<topic>bioluminescence</topic>
<topic>transgenics</topic>
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<title>Genesis</title>
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<topic>Technology Report</topic>
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<identifier type="ISSN">1526-954X</identifier>
<identifier type="eISSN">1526-968X</identifier>
<identifier type="DOI">10.1002/(ISSN)1526-968X</identifier>
<identifier type="PublisherID">DVG</identifier>
<part>
<date>2001</date>
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<caption>vol.</caption>
<number>29</number>
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<title>Developmental Genetics</title>
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<identifier type="ISSN">0192-253X</identifier>
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<date point="end">1999</date>
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<caption>last vol.</caption>
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<identifier type="DOI">10.1002/gene.1014</identifier>
<identifier type="ArticleID">GENE1014</identifier>
<accessCondition type="use and reproduction" contentType="copyright">Copyright © 2001 Wiley‐Liss, Inc.</accessCondition>
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