OperaV1, PascalFrancis, Curation, bibRecord, 000447

Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening

Identifieur interne : 000447 ( PascalFrancis/Curation ); précédent : 000446; suivant : 000448

Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening

Auteurs : Sandra Duffy [Australie] ; Vicky M. Avery [Australie]

Source :

The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 2012.

RBID : Pascal:12-0288826

Descripteurs français

Pascal (Inist)
- Optimisation, Antipaludique, Dépistage, Médecine tropicale, Antiparasitaire.

English descriptors

KwdEn :
- Antimalarial, Medical screening, Optimization, Parasiticide, Tropical medicine.

Abstract

With the increasing occurrence of drug resistance in the malaria parasite, Plasmodium falciparum, there is a great need for new and novel anti-malarial drugs. We have developed a 384-well, high-throughput imaging assay for the detection of new anti-malarial compounds, which was initially validated by screening a marine natural product library, and subsequently used to screen more than 3 million data points from a variety of compound sources. Founded on another fluorescence-based P. falciparum growth inhibition assay, the DNA-intercalating dye 4',6-diamidino-2-phenylindole, was used to monitor changes in parasite number. Fluorescent images were acquired on the PerkinElmer Opera High Throughput confocal imaging system and analyzed with a spot detection algorithm using the Acapella data processing software. Further optimization of this assay sought to increase throughput, assay stability, and compatibility with our high-throughput screening equipment platforms. The assay typically yielded Z'-factor values of 0.5-0.6, with signal-to-noise ratios of 12.

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Le document en format XML

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Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening

Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening

Source :

Descripteurs français

English descriptors

Abstract

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Links to Exploration step

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