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A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.

Identifieur interne : 000243 ( Main/Exploration ); précédent : 000242; suivant : 000244

A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.

Auteurs : Nikko P. Torres [Canada] ; Grant W. Brown

Source :

RBID : pubmed:25916702

English descriptors

Abstract

High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.

DOI: 10.1007/978-1-4939-2596-4_1
PubMed: 25916702


Affiliations:


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Le document en format XML

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<nlm:affiliation>Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.</nlm:affiliation>
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<country>Canada</country>
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<term>Microscopy, Fluorescence (methods)</term>
<term>Saccharomyces cerevisiae (cytology)</term>
<term>Saccharomyces cerevisiae (growth & development)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Stress, Physiological</term>
<term>Time Factors</term>
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{{Explor lien
   |wiki=    Wicri/Musique
   |area=    OperaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:25916702
   |texte=   A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
}}

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